serpine2 human  (Cusabio)

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression is significantly upregulated in COAD. (A) Box plot of SERPINE family genes mRNA expression.in COAD(p < 0.05). (B) Box plot of SERPINE1 and SERPINE2 protein expression in COAD(p < 0.05). (C) Box plot of SERPINE2 mRNA expression among groups of different tumor grades in COAD (p < 0.05).

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression correlates with tumor immunoregulation, division and proliferation. (A) Volcano map of SERPINE2 co-expressed genes in COAD. (B, C) GO and KEGG enrichment analysis of SERPINE2 co-expressed genes obtained from the cBioPortal, the terms p and q < 0.05 were considered to be significantly enriched. Only 20 leading gene sets are displayed in the plot.

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is positively correlated with M2 macrophage infiltration in COAD. (A) Scatter plot showing the correlation of 6 different immune cell types and SERPINE2 expression (p < 0.05). The black line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (B) Scatter plot showing the correlation of CD68+ Marcophage and SERPINE2 expression (p < 0.05). C.Scatter plot showing the correlation of M1/M2 macrophage and SERPINE2 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (p < 0.05).

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is associated with infiltration of CD68 macrophage in COAD patients. (A) The expression of SEPRINE2 in patient tissues was detected by IHC. Adjacent tissue not expressing SERPINE2; tumor tissue strongly expressing SERPINE2. (B) Scatterplot showing the correlation between SERPINE2 and CD68 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the relationship between SERPINE2 and CD68 expression. (C) The expression of SERPINE2 and CD68 in patient tissues were detected by IHC. High levels of SERPINE2 expression are associated with increased expression of CD68. (* p<0.05, **p<0.01, *** p<0.001).

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: The external secretion of SERPINE2 from colorectal cancer cells promotes macrophage polarization to accelerate the development of colorectal cancer. (A) Schema diagram showing that THP-1 cells were treated with PMA, followed by the treatment of SERPINE2 recombinant protein as indicated treatments to obtain activated macrophages. Then tumor cells cocultured with these macrophages. were used to subsequent experiments. (B, C) RT-qPCR and Westen blot displayed SERPINE2 mRNA and Protein expression were upregulated in colorectal cancer cells. (D) The secretion of SERPINE2 in colorectal cancer cells was detected by ELISA. (E) The effect of SERPINE2 recombinant protein on macrophage polarization. (F) RT-qPCR displayed conditioned medium (CM) derived from colorectal cancer cells promotes the upregulation of macrophage M2 markers, compared to normal Intestinal cells. (G) ELISA analysis demonstrated that SERPINE2 secretion was significantly reduced after SERPINE2 knockdown. (H) RT-qPCR displayed conditioned medium with SERPINE2 knockdown down-regulated macrophage M2 markers. (I, J) The conditioned medium from macrophages treated with SERPINE2 recombinant protein promoted cancer cell proliferation (measured by CCK-8 assay) and migration (assessed by Transwell assay. (*p<0.05, **p<0.01, ***p<0.001).

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Recombinant, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Knockdown, CCK-8 Assay, Migration, Transwell Assay

Journal: Journal of Dental Sciences

Article Title: Serpin peptidase inhibitor, clade E, member 2 is associated with malignant progression and clinical prognosis in oral squamous cell carcinoma

doi: 10.1016/j.jds.2023.05.024

Figure Lengend Snippet: mRNA expression with SERPINE2 diagnostic and prognostic values. (A) SERPINE2 mRNA expression levels in 546 normal, tumor, and metastatic head and neck squamous cell carcinoma (HNSCC) tissues. SERPINE2 expressions were higher in tumor tissues than in normal tissues in TNMplot ( P = 2.009e-07). (B) High SERPINE2 mRNA expression was significantly correlated with poor 5-year overall survival in KMPlot ( P = 0.014). (C) Heat map and boxplot of SERPINE2 mRNA expression in 40 OSCC tissue pairs (GSE37991). SERPINE2 is upregulated in human OSCC. Red, upregulated; green, downregulated. Array intensity of SERPINE2 in 40 OSCC tumors compared with their adjacent normal tissues; SERPINE2 intensity is expressed as the log2 ratios. Data are represented as mean ± standard deviation; ∗∗∗∗, P < 0.0001.

Article Snippet: The sections were sequentially incubated at room temperature with monoclonal mouse anti-human SERPINE2 antibody (1:100; #MA5-25936; Invitrogen, Carlsbad, CA, USA) for 60 min, biotinylated secondary antibody for 10 min, and peroxidase-conjugated streptavidin for 10 min.

Techniques: Expressing, Diagnostic Assay, Standard Deviation

Journal: Journal of Dental Sciences

Article Title: Serpin peptidase inhibitor, clade E, member 2 is associated with malignant progression and clinical prognosis in oral squamous cell carcinoma

doi: 10.1016/j.jds.2023.05.024

Figure Lengend Snippet: Representative sections of non-cancer epithelia and OSCC were stained with hematoxylin and eosin and immunostained for SERPINE2. (A) Non-cancer epithelia, (B) weak (1+), (C) moderate (2+) and (D) strong (3+). Scale bar in (A), 100 μm. The scale bar applies to all panels.

Article Snippet: The sections were sequentially incubated at room temperature with monoclonal mouse anti-human SERPINE2 antibody (1:100; #MA5-25936; Invitrogen, Carlsbad, CA, USA) for 60 min, biotinylated secondary antibody for 10 min, and peroxidase-conjugated streptavidin for 10 min.

Techniques: Staining

Journal: Journal of Dental Sciences

Article Title: Serpin peptidase inhibitor, clade E, member 2 is associated with malignant progression and clinical prognosis in oral squamous cell carcinoma

doi: 10.1016/j.jds.2023.05.024

Figure Lengend Snippet: Relationships between the immunoscore of SERPINE2 and clinicopathological parameters in 122 patients.

Article Snippet: The sections were sequentially incubated at room temperature with monoclonal mouse anti-human SERPINE2 antibody (1:100; #MA5-25936; Invitrogen, Carlsbad, CA, USA) for 60 min, biotinylated secondary antibody for 10 min, and peroxidase-conjugated streptavidin for 10 min.

Techniques:

Journal: Journal of Dental Sciences

Article Title: Serpin peptidase inhibitor, clade E, member 2 is associated with malignant progression and clinical prognosis in oral squamous cell carcinoma

doi: 10.1016/j.jds.2023.05.024

Figure Lengend Snippet: High SERPINE2 expression was correlated with worse overall survival rates. Kaplan–Meier survival curve in 122 OSCC patients.

Article Snippet: The sections were sequentially incubated at room temperature with monoclonal mouse anti-human SERPINE2 antibody (1:100; #MA5-25936; Invitrogen, Carlsbad, CA, USA) for 60 min, biotinylated secondary antibody for 10 min, and peroxidase-conjugated streptavidin for 10 min.

Techniques: Expressing

Journal: Journal of Dental Sciences

Article Title: Serpin peptidase inhibitor, clade E, member 2 is associated with malignant progression and clinical prognosis in oral squamous cell carcinoma

doi: 10.1016/j.jds.2023.05.024

Figure Lengend Snippet: Univariate and multivariate analysis of SERPINE2 in 122 oral squamous cell carcinoma patients.

Article Snippet: The sections were sequentially incubated at room temperature with monoclonal mouse anti-human SERPINE2 antibody (1:100; #MA5-25936; Invitrogen, Carlsbad, CA, USA) for 60 min, biotinylated secondary antibody for 10 min, and peroxidase-conjugated streptavidin for 10 min.

Techniques: Expressing

Journal: Journal of Dental Sciences

Article Title: Serpin peptidase inhibitor, clade E, member 2 is associated with malignant progression and clinical prognosis in oral squamous cell carcinoma

doi: 10.1016/j.jds.2023.05.024

Figure Lengend Snippet: SERPINE2 knockdown reduced cell proliferation and invasion in HSC3 and Cal27 cells. (A) SERPINE2 expression levels in OSCC cell lines were evaluated using western blotting. (B) Immunoblotting analysis of SERPINE2 in HSC3 and Cal27 cells transduced with SERPINE2 shRNA and control shRNA (shLuc). (C) Growth curves of HSC3 cells with SERPINE2 knockdown. The migration and invasion of HSC3 cells transduced with SERPINE2 shRNA or control vector were assessed using Transwell assays. (D) Growth curves of Cal27 cells with SERPINE2 knockdown. Transwell assays were performed to analyze the migration and invasion of Cal27 cells transduced with SERPINE2 shRNA or a control vector. bar: SEM; ∗ P < 0.05; ∗∗ P < 0.001.

Article Snippet: The sections were sequentially incubated at room temperature with monoclonal mouse anti-human SERPINE2 antibody (1:100; #MA5-25936; Invitrogen, Carlsbad, CA, USA) for 60 min, biotinylated secondary antibody for 10 min, and peroxidase-conjugated streptavidin for 10 min.

Techniques: Expressing, Western Blot, Transduction, shRNA, Migration, Plasmid Preparation

Journal: Respiratory Research

Article Title: High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma

doi: 10.1186/s12931-020-01597-5

Figure Lengend Snippet: Microscopic images of spiral array specimens. Low power fields of a H&E staining, c SERPINE2 positive staining, and e SERPINE2 negative staining and high power fields of b H&E staining, d SERPINE2 positive staining, and f SERPINE2 negative staining are shown

Article Snippet: Anti-human SERPINE2 antibody (Lot no. 00014298, Proteintech, Rosemont, IL, USA) was used as the primary antibody.

Techniques: Staining, Negative Staining

Journal: Respiratory Research

Article Title: High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma

doi: 10.1186/s12931-020-01597-5

Figure Lengend Snippet: Univariate analysis

Article Snippet: Anti-human SERPINE2 antibody (Lot no. 00014298, Proteintech, Rosemont, IL, USA) was used as the primary antibody.

Techniques:

Journal: Respiratory Research

Article Title: High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma

doi: 10.1186/s12931-020-01597-5

Figure Lengend Snippet: Multivariate analysis

Article Snippet: Anti-human SERPINE2 antibody (Lot no. 00014298, Proteintech, Rosemont, IL, USA) was used as the primary antibody.

Techniques:

Journal: Respiratory Research

Article Title: High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma

doi: 10.1186/s12931-020-01597-5

Figure Lengend Snippet: SERPINE2 expression and knockdown effect of SERPINE2 . a SERPINE 2 expression in lung cancer cell lines was normalized to BEAS-2B. b , c The efficacies of siRNA in A549 and PC9 cells were analyzed by qRT-PCR (B, relative to negative control) and western blotting ( c ). nc, negative control; #1, siRNA#1; #2, siRNA#2

Article Snippet: Anti-human SERPINE2 antibody (Lot no. 00014298, Proteintech, Rosemont, IL, USA) was used as the primary antibody.

Techniques: Expressing, Knockdown, Quantitative RT-PCR, Negative Control, Western Blot

Journal: Respiratory Research

Article Title: High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma

doi: 10.1186/s12931-020-01597-5

Figure Lengend Snippet: Effect of SERPINE2 knockdown on cell number. The results of the cell number assay are shown. nc, negative control; #1, siRNA#1; #2, siRNA#2

Article Snippet: Anti-human SERPINE2 antibody (Lot no. 00014298, Proteintech, Rosemont, IL, USA) was used as the primary antibody.

Techniques: Knockdown, Negative Control

Journal: Respiratory Research

Article Title: High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma

doi: 10.1186/s12931-020-01597-5

Figure Lengend Snippet: Effect of SERPINE2 knockdown on apoptosis. Western blotting analysis of apoptosis-related proteins at the indicated time points after Fas ligand stimulation is shown. A549 (upper) and PC9 (lower) cells pretreated with SERPINE2 siRNA were stimulated with Fas ligand (200 ng/ml). nc, negative control; #2, siRNA#2

Article Snippet: Anti-human SERPINE2 antibody (Lot no. 00014298, Proteintech, Rosemont, IL, USA) was used as the primary antibody.

Techniques: Knockdown, Western Blot, Negative Control